Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lucas B. Harrington
Date created: 2012-08-12 11:00:00
Date modified: 2015-05-08 01:13:44
Extended psbA2 with Zeaxanthin 7,8-cleavage dioxygenase (ZCD)
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K906100_sequence (Version 1) |
Description
This translational unit is designed to constitutivly express Zeaxanthin 7,8-cleavage dioxygenase (ZCD) in synechocystis. PsbA2 can be removed from this part and utilized with an alternative part by using NdeI to cut between the end of psbA2 and the start codon of ZCD. The ORF to be attached to this promoter should be made to have the same restriction enzyme site (possibly added by PCR) at its 5' end. This will allow for spacing between the regulatory features of psbA2 and the ORF to parallel that of the native use of the promoter.Notes
The point mutation (A->T) enables the use of NdeI as mentioned on the main page.Source
psbA2 is from the genomic sequence preceding the photosystem II D1 protein coding region in Synechocystis spp. 6803. The modified version of ZCD has been codon optimized for the species Synechocystis 6803.| Access | Instance | Definition |
| public public | BBa_K906006 BBa_K906000 | BBa_K906006 BBa_K906000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K906006 BBa_K906000 | 1,200 201,1310 | BBa_K906006 BBa_K906000 |