Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dong-hui Choe, Soo-in Lee
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:44
Mycobacterium Phage Bxb1 gp47, DNA excisionase
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K907001_sequence (Version 1) |
Description
This part is the protein coding sequence which encodes DNA integrase of Mycobacterium phage Bxb1.The Bxb1 integrase is a DNA recombinase, more precisely a member of serine integrase family. It recognizes specific sequences, called attB and attP, and then integrate, invert, or excise depend on orientations of recognition sequences.
We used this integrase to invert specific sequence contained in plasmid. This protein is well expressed in E.coli(strain MG1655).
When it inverts DNA, the attB and attP sequences are changed into attL and attR, as common DNA recombinases do. Another protein called Bxb1 excisionase interacts with integrase and that complex flips back inverted DNA into original sequence regenerating attB and attP sequences.
Notes
Internal restriction endonuclease site is changed(two PstI sites, one XbaI sites) without changing amino acid sequence.Source
This part is derived by Mycobacterium phage Bxb1. And synthesized by Bioneer.http://www.ncbi.nlm.nih.gov/protein/NP_075302.1
| Sequence Annotation | Location | Component / Role(s) |
| Bacteriophage Bxb1 excisionase CDS | 1,768 | CDS feature/cds |