Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dong-hui Choe, Soo-in Lee
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:44
Binary signal generator, RBS(reverse) - attB - Promoter - attP - RBS
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K907002_sequence (Version 1) |
Description
Dual phase protein generator promoter reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBSThis part is composed of 3 elements.
1. RBS: BBa_B0034, upsteam one is reversed
2. Promoter: BBa_J23119, Bacterial constitutive promoter
3. att site: Recognition site for Mycobacteriophage Bxb1 integrase/excisionase
We designed this part to generate two different proteins one at a time.
At the first state, this device promotes transcription and translation of downstream gene because its promoter orientation.
When Mycobacteriophage Bxb1 integrase recognizes and inverts sequence flanking with attB and attP sequence, promoter orientation reversed. Then this device promotes transcription and translation of upstream gene(must be reversed at the construction step).
This device is tested by GFP and mRFP attached construct. See BBa_K090700N and BBa_K090700N
Notes
NoSource
Promoter and RBS are derived from BBa_J23119 and BBa_B0034, respectively.Whole construct was de novo synthesized by Bioneer.
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0034, reverse BBa_B0034 attB(Bxb1 Int) attP(Bxb1 Int) BBa_J23119 | 1,12 163,174 19,68 104,156 69,103 | feature/rbs ribosome_entry_site ribosome_entry_site feature/rbs sequence_feature feature/misc sequence_feature feature/misc promoter feature/promoter |