Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dong-hui Choe, Soo-in Lee
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:44
Binary signal generator, promoter reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K907003_sequence (Version 1) |
Description
Dual phase protein generator promoter reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBSThis part is composed of 3 elements. 1. RBS: BBa_B0034, upsteam one is reversed 2. Promoter: BBa_J23119, Bacterial constitutive promoter 3. att site: Recognition site for Mycobacteriophage Bxb1 integrase/excisionase We designed this part to generate two different proteins one at a time.
At the first state, this device promotes the transcription and translation of downstream gene because of its promoter orientation. When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence flanking with attB and attP sequence, promoter orientation reversed. Then this device promotes the transcription and translation of upstream gene(must be reversed at the construction step).
This device is tested by GFP and mRFP attached construct. See BBa_K090700N and BBa_K090700N
Notes
NoSource
Promoter and RBS are derived from BBa_J23119 and BBa_B0034, respectively. Whole construct was de novo synthesized by Bioneer.| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0034(reverse) BBa_B0034 attB(Bxb1 Int) attP(Bxb1 Int) BBa_J23119(reverse) | 1,12 163,174 19,68 104,156 69,103 | ribosome_entry_site feature/rbs feature/rbs ribosome_entry_site sequence_feature feature/misc feature/misc sequence_feature feature/promoter promoter |