Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gintautas Vainorius
Date created: 2012-09-22 11:00:00
Date modified: 2015-05-08 01:13:45
TetR DNA binding domain containing BamHI site for protein fusions
| Types | DnaRegion |
| Roles | Protein_Domain polypeptide_domain |
| Sequences | BBa_K909007_sequence (Version 1) |
Description
Truncated version of tetracycline repressor TetR, consisting of 1 ??? 127 amino acids, containing DNA binding domain. The rest of the structure, 8-10 helixes responsible for tetR dimerization, were removed in order to keep tetR-DBD in monomer form and prevent from efficient DNA binding and transcription repression.In addition, we introduced BamHI site at C terminus for an easy in tetR-DBD frame fusions with other proteins. Thus, it can be used in two hybrid systems for both, homo and hetero dimerizations, also one can use these fusions to turn protein-protein interaction into the repression of transcription.
Notes
Deletion of 8-10 helixes might lower the stability of protein, also TetR-DBD is no longer responsive to anhydrotetracyclineSource
E. coli Tn10 transposon| Sequence Annotation | Location | Component / Role(s) |
| BamHI restriction site | 382,387 | sequence_feature feature/misc |