Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gintautas Vainorius
Date created: 2012-09-22 11:00:00
Date modified: 2015-05-08 01:13:45
tetR-DBD-UVR8 fusion protein
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K909008_sequence (Version 1) |
Description
Tetracycline repressor DNA binding domain fusion with truncated version of UVR8 (tetR-DBD-UVR8).UVR8 is a UV-B sensing receptor in plants necessary for UV-B protection. In dark state UVR8 forms a dimer which is monomerized by the UV-B light (280-315 nm). A truncated version of UVR8 (14-440 amino acids) was fused with tetR DNA binding domain (tetR-DBD), lacking of dimerization domain. Such mutant shows no repression of Ptet promoter, however fusion with dimerizing protein (UVR8) returns tetR-DBD activity. Later UV-B exposure breaks UVR8 dimer and releases tetR-DBD-UVR8 from Ptet, activating transcription. Thus, this tetR-DBD-UVR8 can be used as a one step UV-B on switch.
Notes
Contains two illegal PstI sites in UVR8 coding region and BamHI site was used for protein fusionSource
tetR-DBD was cloned from E.coli tetracyclin repressor tetR from transposon Tn10cDNA of UVR8 was provided by Roman Ulm, University of Geneva.