Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Deborah Huber
Date created: 2012-09-22 11:00:00
Date modified: 2015-05-08 01:13:45
YcgZ promoter with multiple BluR (YcgE) operator sites
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K909010_sequence (Version 1) |
Description
Escherichia coli senses blue light via the BLUF-EAL protein BluF (YcgF). The degenerate EAL domain of BluF does not have cyclic-di-GMP phosphodiesterase activity, but BluF directly antagonizes the MerR-like repressor BluR (YcgE), which leads to expression of the ycgZ-ymgABC operonNotes
Based on the paper (Tschowri et al., 2012; Mol. Microbiol: 1-14) published in July 2012 in that they showed the binding site of BluR to the YcgZ promoter, more BluR binding sites were introduced to the YcgZ promoter to decrease leakiness of the promoter and therefore increase the repression by BluR.Source
K238013 as template for PCRTschowri et al., 2009; Genes Dev 23: 522??? 534
Tschowri et al., 2012; Mol. Microbiol: 1-14; doi:10.1111/j.1365-2958.2012.08147.x
| Sequence Annotation | Location | Component / Role(s) |
| BluR operator site BluR operator site -35 -10 BluR operator site BluR operator site | 26,31 40,44 28,33 52,57 55,60 69,73 | operator feature/operator operator feature/operator promoter feature/promoter promoter feature/promoter operator feature/operator operator feature/operator |