BBa_K909016

BBa_K909016 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K909016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sandro Kundert

Date created: 2012-09-23 11:00:00

Date modified: 2015-05-08 01:13:45

PR promoter with two operator sites: cI OR1, cI OR2 and and LacI O1/O2

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K909016_sequence (Version 1)

Description

The promoter is derived from the PR (lambda phage) promoter. The -35 and -10 regions as well as the cI operator sites are maintained while the sequence downstream of the -10 region is replaced by the operator LacO1.

Notes

This double negative hybrid promoter is only active, if cI AND lacI are absent. It functions as a biological NOR gate.

Source

de-novo design

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
PR -35	20,25	feature/promoter, promoter
PR -10	43,48	promoter, feature/promoter
cI OR2	6,25	operator, feature/operator
cI OR1	26,48	feature/operator, operator
lac O1/O2	52,82	feature/operator, operator

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K909016/1

Member of these Collections

• iGEM Parts Registry