Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oliver Meacock
Date created: 2012-09-06 11:00:00
Date modified: 2015-05-08 01:13:45
Magnesium sensitive riboswitch
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_K911001_sequence (Version 1) |
Description
Riboswitch that acts as a regulatory element, truncating transcripts when magnesium is not bound to the RNA. Four Mg2+ binding sites exist, giving this part significantly switch-like behavior. Binding of these sites by Mg2+ results in compaction of the regulatory region of the riboswitch, which in its unbound state acts as an anti-terminator. Loss of this anti-terminator activity results in the activity of a downstream terminator (included in this sequence) which terminates transcription of the gene.May also be affected by other divalent ions, such as manganese.
Notes
Original paper mentioned 8 codon substitution of the downstream LacZ reporter gene with the first 8 codons of the MgtE gene (which is the native gene which this riboswitch controls). Our construct used LacI as the downstream regulated sequence, which requires its N-terminal domain to bind to DNA. Sequences both with and without this substitution have been submitted. This copy does not contain the substitution.Source
Bacillus Subtilis Genome| Sequence Annotation | Location | Component / Role(s) |
| Terminator M-box | 284,299 95,250 | sequence_feature feature/misc feature/misc sequence_feature |