BBa_K911003

BBa_K911003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K911003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oliver Meacock
Date created: 2012-09-11 11:00:00
Date modified: 2015-05-08 01:13:45

Fluoride Sensitive Riboswitch



Types
DnaRegion

Roles
engineered_region

Measurement

Sequences BBa_K911003_sequence (Version 1)

Description

Riboswitch that is highly sensitive to the F- ion. We have characterized this part in three different chassis: TOP10 e.coli, Laboratory strain bacillus subtilis and a strain of bacillus subtilis with its normal fluoride riboswitch knocked out (kindly provided by the Breaker lab in Yale). Results of Miller assays for these three chassis are shown below.

Notes

Because this is a positive regulator (it ceases transcriptional termination in the presence of F- ions), it was decided that it should be placed upstream of a direct reporter, in this case lacZ as our reporter.

Source

Bacillus Cereus Genome

Sequence Annotation Location Component / Role(s)
Lysine promoter
Fluoride riboswitch
1,43
61,139
promoter feature/promoter
feature/misc sequence_feature
igem#experience
Works
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K911003/1