Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Thomas Whittaker
Date created: 2012-09-22 11:00:00
Date modified: 2015-05-08 01:13:45
Non-standard backbone for luciferase construct
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K911006_sequence (Version 1) |
Description
This is the sequence of the non-standard backbone that our luciferase construct, BBa_k911004, was synthesised in. It could not be assembled in the high copy number standard psb1c3 backbone due to toxicity issues. Attempts to assemble the 9kb insert into psb3c5, a low copy number biobrick plasmid have so far been unsuccessful.The sequence of the low copy number custom backbone is provided here as a reference.
Notes
We did not design this sequence - however, it was chosen to assemble in as it is maintained at a low copy number, avoiding fatal toxicity.Source
synthesis by DNA 2.0| Sequence Annotation | Location | Component / Role(s) |
| Biobrick Suffix Element #4.2 bla txn terminator rpn txn terminator Element #5 Element #6 p15a ori KanR Pamp rrnB1 B2 txn terminator Element #3.1 Biobrick Prefix | 1,21 26,97 98,398 405,518 399,404 519,580 581,1407 1412,2221 2222,2399 2400,2574 2575,2645 2650,2671 | feature/misc sequence_feature feature/misc sequence_feature feature/stop stop_codon stop_codon feature/stop feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/stop stop_codon feature/misc sequence_feature sequence_feature feature/misc |