BBa_K911008

BBa_K911008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K911008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stuart Bell
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:45

Fast Germination (B.subtilis)



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K911008_sequence (Version 1)

Description

This part upregulates an operon responsible for germination rate. Bacillus subtilis spores germinate in response L-Alanine. Up-regulation of the spoVA operon increases germination rate in response to L-Alanine. The promoter for the B.subtilis sspB gene (PsspB) is more active than the endogenous spoVA promoter. It is also active during germination. Expression of the spoVA operon under PsspB increases the germination rate.

This construct consists mainly of the sspB promoter followed by the first 354bp of the spoVA operon (first 354bp of the spoVAA gene). Since B.subtilis exhibits accurate and efficient homologous recombination, a single cross-over event between the spoVAA region in the BioBrick and the endogenous spoVAA sequence inserts the sspB promoter upstream of the spoVA operon.

Notes

The design of this BioBrick is based on a plasmid (pPS3393)from Wang et al. (2011)

Wang, G., Yi, X., Li, Y.-Q., Setlow, P. Germination of individual Bacillus subtilis spores with alterations in the GerD and SpoVA proteins, which are important in spore germination(2011) J. Bacteriology, 193 (9), pp. 2301-2311.

Source

spoVAA and PsspB from B.subtilis Genome.

Sequence Annotation Location Component / Role(s)
PsspB (Highly active germination specific promoter)
SPOVAA insertion sequence
20,104
199,552
promoter feature/promoter
sequence_feature feature/misc
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K911008/1