BBa_K912031

BBa_K912031 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K912031
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Nicholas
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:45

pH Inducible Lysis System(m)



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K912031_sequence (Version 1)

Description

This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector meant to self lyse in high chloride and low pH conditions. The device is designed to lyse in the presence of high chloride concentrations. The Pgad promoter in front of the lysis cassette is activated by the combination of high extracellular hydrogen ion concentration and the presence of high intracellular chloride ion concentration.

Notes

None besides designing primers properly

Source

Lysis:registry
CP/RBS: registry
clc-ec1: PCR from genome
Pgad: PCR from vector from CUHK 2011 team

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K912031/1