Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yuan Tao
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:45
pH Inducible GFP system (m)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K912036_sequence (Version 1) |
Description
This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector. This particular part was constructed in order to test the to make sure that the Pgad/clc system works correctly. We hoped that it would be easier to see GFP for assay purposes. The original device is designed to lyse in the presence of high chloride concentrations. The Pgad promoter in front of the lysis cassette is activated by the combination of high extracellular hydrogen ion concentration and the presence of high intracellular chloride ion concentration.The chloride antiporter was promoted by a strong promoter.
Notes
noneSource
GFP:registry CP/RBS: registry clc-ec1: PCR from genome Pgad: PCR from vector from CUHK 2011 team| Access | Instance | Definition |
| public public public public public public | BBa_K559011 BBa_E0040 BBa_J23110 BBa_K912005 BBa_B0010 BBa_B0012 | Pgad GFP BBa_J23110 BBa_K912005 BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K559011 BBa_E0040 BBa_J23110 BBa_K912005 BBa_B0010 BBa_B0012 | 1,1251 1258,1977 1986,2020 2027,3448 3457,3536 3545,3585 | Pgad GFP BBa_J23110 BBa_K912005 BBa_B0010 BBa_B0012 |