BBa_K914000

BBa_K914000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K914000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jean Cury

Date created: 2012-09-17 11:00:00

Date modified: 2015-05-08 01:13:45

pLac-supD-T

Details

• Types: DnaRegion
• Roles: mature_transcript_region; RNA
• Sequences: BBa_K914000_sequence (Version 1)

Description

This part is composed of a tRNA amber suppressor, supD (K228001), which is under the control of pLac (R0011). A terminator is added (B1006, but the sequence is not the exact one, it's not a problem here).
This part is used to allow the expression of gene that have one or many amber codon instead of a Serine. Adding IPTG could be usefull when there are to many amber codon in the cell.

Notes

The promoter is the last part added, meaning that from the previous intermediate (soon available), we will be able to change the promoter easily.

Source

This part is made from the 3 Biobricks previously describe : R0011 - K228001 - B1006

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0011	1,54	feature/BioBrick, engineered_region
lac O1	3,19	operator, feature/operator
-35	20,25	feature/promoter, promoter
lac O1	26,42	feature/operator, operator
-10	43,48	promoter, feature/promoter
BBa_K228001	64,199	feature/BioBrick, engineered_region
BBa_B1006 modified	208,241	feature/BioBrick, engineered_region
modified thr terminator	217,238	stem_loop, feature/stem_loop

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K914000/1

Member of these Collections

• //biosafety/semantic_containment
• iGEM Parts Registry