Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Denis Samuylov
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:45
Meganuclease I-SceI controlled by pLac
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K914005_sequence (Version 1) |
Description
I-SceI homing endonuclease expression is controlled by pLac promoter. Expression is induced with IPTG if LacI is present in the cell. I-SceI doesn't have an LVA degradation tag.Notes
The pLac promoter is leaky.Source
Promoter pLac and RBS was taken from an iGEM distribution plate (BBa_R0011 and BBa_B0032). Meganuclease was amplified from the chromosome of SMR6316 E.Coli strain (Dr. Rosenberg).| Access | Instance | Definition |
| public public public | BBa_R0011 BBa_B0032 BBa_K914006 | lacI+pL BBa_B0032 BBa_K914006 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0011 BBa_B0032 BBa_K914006 | 1,54 64,76 83,794 | lacI+pL BBa_B0032 BBa_K914006 |