BBa_K914008

BBa_K914008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K914008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Denis Samuylov
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:46

Meganuclease I-SceI controlled by pRha



    • Details

    Types
    DnaRegion

    Roles
    Generator

    engineered_region

    Sequences BBa_K914008_sequence (Version 1)

    Description

    I-SceI homing endonuclease expression is controlled by pRha promoter. Expression is induced with L-rhamnose. I-SceI doesn't have an LVA degradation tag.

    Notes

    The system is leaky and meganuclease cut RS with high efficiency even without pRha induction.

    Source

    Promoter pRha was amplified from the plasmid pJOE3075 (Dr. Altenbuchner). RBS was taken from an iGEM distribution plate (BBa_B0032). Meganuclease was amplified from the chromosome of SMR6316 E.Coli strain (Dr. Rosenberg).

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