BBa_K914009

BBa_K914009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K914009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jean Cury
Date created: 2012-09-20 11:00:00
Date modified: 2015-05-08 01:13:46

P1003* Ser133->Amber Codon



Types
DnaRegion

Roles
Translational_Unit

engineered_region

Sequences BBa_K914009_sequence (Version 1)

Description

The single mutation avoid the expression of the P1003 gene, that prevent the expression of a kanamycin resistance.
This mutation is rescue with the tRNA amber suppressor supD (BBa_K914000), or any genotype relative to sup44 . The idea here is to prevent the kanamycin gene resistance in case horizontal gene transfer.
Actually it would be better with another mutation.

Notes

I made the single point mutation with a quick change directed mutagenesis kit.

Source

this part comes from BBa_P1003

Sequence Annotation Location Component / Role(s)
BBa_P1003
kanamycin resistance
Ser133->Amber codon
1,967
149,967
546,546
feature/BioBrick engineered_region
feature/cds CDS
point_mutation feature/s_mutation
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BBa_K914009/1