View

BBa_K914014

BBa_K914014 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K914014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Dylan Iverson

Date created: 2012-10-24 11:00:00

Date modified: 2015-05-08 01:13:46

Pbad/AraC RBS LacI[LVA(-)] Plac GFP

Back

Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K914014_sequence (Version 1)

Description

Used to characterize Paris Bettencourt 2012 delay system. When arabinose is no longer present LacI is able to leave by dilution eventually allowing GFP expression.

Notes

LVA tag was removed from LacI by PCR. The delay was not functional with fast-degrading LacI.

Source

Regristry kits

Components

Access	Instance	Definition
public public public public public public public public public	BBa_I13458 BBa_I13453 BBa_B0034 BBa_K143033 BBa_K091110 BBa_B0032 BBa_E0040 BBa_B0010 BBa_B0012	BBa_I13458 BBa_I13453 BBa_B0034 LacI BBa_K091110 BBa_B0032 GFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I13458 BBa_I13453 BBa_B0034 BBa_K143033 BBa_K091110 BBa_B0032 BBa_E0040 BBa_B0010 BBa_B0012	1,1114 1123,1252 1261,1272 1279,2364 2373,2428 2437,2449 2456,3175 3184,3263 3272,3312	BBa_I13458 BBa_I13453 BBa_B0034 LacI BBa_K091110 BBa_B0032 GFP BBa_B0010 BBa_B0012

Other Properties

igem#experience	Works
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K914014/1