BBa_K916004

BBa_K916004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K916004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Joseph Elsherbini

Date created: 2012-10-01 11:00:00

Date modified: 2015-05-08 01:13:46

Leucine Zipper CGFP translational fusion

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K916004_sequence (Version 1)

Description

This is for use with the leucine zipper NGFP fusion (part BBa_K916003). When both protein products are expressed, they will produce fluorescence due to the dimerization of the leucine zipper domains.

Notes

This part is compatible in its current form with most biobrick assembly methods. There is no promoter or RBS with this part, so one can select the desired promoter and the strength of the RBS from other parts in the registry.

Source

The CGFP fragment came from a plasmid (pMRBAD-z-NGFP) provided by Dr. Lynne Regan at Yale University.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
NcoI site	1,6	sequence_feature, feature/misc
zipper CGFP fusion	3,365	CDS, feature/cds
start	3,5	start_codon, feature/start
leucine zipper domain	3,98	sequence_feature, feature/misc
CGFP fragment	108,362	feature/misc, sequence_feature
stop	363,365	feature/stop, stop_codon
AatII site	101,106	sequence_feature, feature/misc

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K916004/1

Member of these Collections

• iGEM Parts Registry