Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris French
Date created: 2012-06-18 11:00:00
Date modified: 2015-05-08 01:13:46
cscA sucrose hydrolase from E. coli 157:H7 Sakai
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K917000_sequence (Version 1) |
Description
The is the gene cscA (Ecs3243) from Escherichia coli O157:H7 strain Sakai, encoding a sucrose hydrolase gene. K12-derived laboratory strains of E. coli generally can not use sucrose as a carbon source, but it has been reported that expression of this gene alone from the related strain E. coli W is sufficient to allow good growth with sucrose as sole carbon source. This BioBrick includes the native ribosome binding site (overlapping the scar sequence).Notes
Due to the pathogenic nature of the source strain, purified genomic DNA was obtained from a veterinary laboratory to use as a PCR template, and the E. coli O157:H7 strain was never present in viable form in our laboratory. Note that the native RBS is included.Source
Escherichia coli O157:H7 strain Sakai| Sequence Annotation | Location | Component / Role(s) |
| start codon stop codon RBS | 11,13 1442,1444 1,2 | feature/start start_codon feature/stop stop_codon ribosome_entry_site feature/rbs |