Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jakub Krakowiak
Date created: 2012-08-09 11:00:00
Date modified: 2015-05-08 01:13:46
S. oneidensis decaheme cytochrome c electron export system mtrCAB
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K917007_sequence (Version 1) |
Description
This part consists of three genes from the native gene cluster from Shewanella oneidensis MR-1 with native RBSs: MtrA is a decaheme cytochrome c located between inner and outer membrane; MtrB is aa transmembrane beta-barrel responsible for facilitating contact between MtrA and MtrC; MtrC (alternative name: OmcB) is a decaheme cytochrome c attached to the outer membrane. Together these proteins are able to form an extracellular electron export system. (Jensen et al, 2010, PNAS vol. 107 no. 45 19213-19218; doi: 10.1073/pnas.1009645107)Notes
Genes were obtained by PCR amplification of S. oneidensis MR-1 gene cluster.MtrA and MtrC contained PstI restriction sites that had to be removed by site-directed mutagensis
Source
Shewanella oneidensis MR-1 chromosomal DNA| Sequence Annotation | Location | Component / Role(s) |
| Start codon Stop codon PstI site T>A PstI site G>A RBS | 41,43 5230,5232 1834,1834 2191,2191 31,36 | start_codon feature/start feature/stop stop_codon sequence_alteration feature/mutation feature/mutation sequence_alteration ribosome_entry_site feature/rbs |