Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jakub Krakowiak
Date created: 2012-08-09 11:00:00
Date modified: 2015-05-08 01:13:46
S. oneidensis decaheme cytochrome c mtrA
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K917008_sequence (Version 1) |
Description
This part consists of Shewanella oneidensis MR-1 gene mtrA with native RBS. MtrA is a decaheme cytochrome c responsible for electron transport within periplasm and was demonstrated to reduce soluble iron (III) to iron (II). (Jensen et al., 2010, PNAS vol. 107 no. 45 19213-19218; doi: 10.1073/pnas.1009645107)Notes
Gene was obtained by PCR amplification of S. oneidensis MR-1 gene.MtrA contains a PstI site that had to be removed by site-directed mutagenesis.
Source
Shewanella oneidensis MR-1 chromosomal DNA| Sequence Annotation | Location | Component / Role(s) |
| PstI site G>A Start codon Stop codon RBS | 89,89 23,25 1022,1024 13,16 | sequence_alteration feature/mutation start_codon feature/start stop_codon feature/stop feature/rbs ribosome_entry_site |