| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K925004_sequence (Version 1)
|
Description
This part consists of a glutathione S-transferase (GST) protein that has been modified to include a short peptide sequence allowing it to bind to nickel. The purified protein will bind to both GST and nickel, giving a double ended tag and a mechanism by which to recover nickel from solution.
Notes
During the design of this part our main consideration was the difficulty of inserting a very small peice of DNA into the pGEX-6P-1 plasmid. This created difficulty as we could not do diagnostic digests and our vector was more likely to self-ligate.
Source
This part was biologically synthesised.
