BBa_K925005

BBa_K925005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K925005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Paul Lockhart
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:46

PdTagGST



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K925005_sequence (Version 1)

Description

This part produces functioning glutathione S-transferase (GST) protein that has a short petide sequence that binds palladium attached to the end of it. This allows the part to function as a palladium scavenger as the GST end can be bound to GST beads, while the Palladium binding end is free to bind to any free Pd in solution.

Notes

When designing the sequence for this part we had to consider the vector we would use as we required one that already contained GST. This allowed us to design primers that would anneal to each other to make the short peptide sequence and use this as our insert.

Source

This part was biologically synthesised.

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K925005/1