BBa_K929100

BBa_K929100 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K929100
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Potsdam Bioware 2012 iGEM
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:46

Anti-EGFR scFv425



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K929100_sequence (Version 1)

    Description

    The single chain fragment (scFV425) specifically binds to the epidermal growth factor receptor (EGFR) domain 3. The part has a shortened N-terminal FLAG-tag sequence of five amino acids (DYKDE) for purification and detection.

    Notes

    PstI mutations originally occurring in the initial scFv425 sequence were eliminated by mutagenesis PCR.

    Source

    Plasmid pKMEF425bla

    Sequence Annotation Location Component / Role(s)
    Flag tag
    scFv425
    		1,13
    14,741
    		sequence_feature feature/misc
    CDS feature/cds

    igem#experience
    None
     
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K929100/1
     

    Attachments

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