BBa_K929103

BBa_K929103 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K929103
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Potsdam Bioware 2012 iGEM
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:46

B-cell transmembrane region with mCherry and TEV site, framed by LoxP



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K929103_sequence (Version 1)

Description

This part represents the switchable membrane anchoring region of or antibody construct (BBa_K929107).

Notes

Gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus).

Source

TEV: TEV Protease recognition site according to life technologies manual (AcTEV TM Protease),gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)

LoxP sites: two for Cre mediated recombination, gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)

Transmembrane domain: transmembrane domain of B-cell receptor with glycine-serine linker (BBa_K157010),gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)

Reportergene: mcherry, gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)

Sequence Annotation Location Component / Role(s)
TEV recognition site
LoxP site
B-cell receptor transmembrane domaine with linker
mCherry
misc
1,21
22,55
58,144
145,852
856,890
feature/misc sequence_feature
sequence_feature feature/misc
feature/misc sequence_feature
CDS feature/cds
feature/misc sequence_feature
igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K929103/1