BBa_K929104

BBa_K929104 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K929104
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Potsdam Bioware 2012 iGEM
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:47

Anti-GFP Nanobody



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K929104_sequence (Version 1)

Description

The green fluorescent protein (GFP)-nanobody is a single-chain VHH antibody domain developed with specific binding activity against GFP and shows a Kd value of 1.4 nM. Its CDR3 loop is very short and has significantly fewer contacts with the GFP ligand compared to other nanobodies. Furthermore, the shortness of this CDR3 loop leads to the exposure of the framework 2 region, which has a major contribution to the binding with GFP.

Notes

Gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus).

Source

Nanobody: anti-GFP nanobody taken from Protein Data Bank(PDB: 3OGO), gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)

Sequence Annotation Location Component / Role(s)
anti-GFP nanobody
1,348
feature/cds CDS
igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K929104/1