Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Potsdam Bioware 2012 iGEM
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:47
Anti-GFP Nanobody
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K929104_sequence (Version 1) |
Description
The green fluorescent protein (GFP)-nanobody is a single-chain VHH antibody domain developed with specific binding activity against GFP and shows a Kd value of 1.4 nM. Its CDR3 loop is very short and has significantly fewer contacts with the GFP ligand compared to other nanobodies. Furthermore, the shortness of this CDR3 loop leads to the exposure of the framework 2 region, which has a major contribution to the binding with GFP.Notes
Gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus).Source
Nanobody: anti-GFP nanobody taken from Protein Data Bank(PDB: 3OGO), gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)| Sequence Annotation | Location | Component / Role(s) |
| anti-GFP nanobody | 1,348 | feature/cds CDS |