| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K929302_sequence (Version 1)
|
Description
AID is known to be responsible for somatic hypermutation and the class-switch recombination of immunoglobulin in B cells. This enzyme of 28 kDa originally occurs in B cells but does also show activity after transfection into CHO cells. AID induces the deamination of cytidine to uridine at actively transcribed single strand DNA. The replacement of cytidine by uridine leads to a mismatch during DNA replication and integrates a single base substitution predominantly in the immunoglobulin genes.
Notes
AID was amplified with primers which contains thiophosphates at 5' end and insert in Potsdam Standard Backbone using PLIcing method.
Source
BBa_K103001
