Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris Clifford
Date created: 2012-09-28 11:00:00
Date modified: 2015-05-08 01:13:47
SBP - TBP
| Types | DnaRegion |
| Roles | Intermediate engineered_region |
| Sequences | BBa_K931005_sequence (Version 1) |
Description
The starch-binding protein gene (CBM21) and the thiamine-binding protein gene (ThiT) were placed in sequence with each other in that ThiT comes before CBM21. CBM21 comes from R. oryzae. It is part of an operon that codes for a two-domain enzyme, one of which binds starch molecules. This can be used to bind starch residues that are found in rice. ThiT comes from E.coli and plays a crucial role in the thiamine transport system by binding the thiamine molecule. The resulting protein complex from these two genes has a starch-binding domain and a thiamine binding domain that bind their specific substrates.Notes
The two genes were placed in sequence of each other with the thiamine-binding protein gene preceding the starch-binding protein gene. The linker sequence between each element is ACTAGA.Source
The two genes come from separate organisms. The thiamine-binding protein gene comes from the genomic DNA of E. coli and the starch-binding protein gene comes from the genomic DNA of R. oryzae.| Access | Instance | Definition |
| public public | BBa_K931000 BBa_K931004 | BBa_K931000 BBa_K931004 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K931000 BBa_K931004 | 1,362 369,1349 | BBa_K931000 BBa_K931004 |