BBa_K936012

BBa_K936012 Version 1

Component

This part has been discontinued.

Please consider using BBa_J32015 as a replacement.

Source: http://parts.igem.org/Part:BBa_K936012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mattan Hamou

Date created: 2012-07-23 11:00:00

Date modified: 2015-05-08 01:13:47

Leader sequence that brings protein to periplasm

Details

• Types: DnaRegion
• Roles: Signalling; engineered_region
• Sequences: BBa_K936012_sequence (Version 1)

Description

PelB is a sequence of amino acids that when attached to a protein coding sequence allows the cell to bring the protein to the periplasm of the cell. In our project pelB is being used in order to allow our cutinase enzyme to be brought to the periplasm of the cell. The ultimate goal for this sequence is to aid the cell in secreting our protein into the media in order to show degradation of PET. Our method of getting the enzyme to a high level of secretion is to overproduce it in the cell using the pelB sequence to bring the protein to the periplasm allowing for secretion.

Notes

Adding the pelB sequence in front of our protein coding sequence in order to make a pelB-LC-Cutinase construct.

Source

Artificial sequence

Other Properties

• igem#experience: None
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• isReplacedBy: BBa_J32015/1
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K936012/1

Member of these Collections

• iGEM Parts Registry