BBa_K936033

BBa_K936033 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K936033
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sergio Castro Waqued
Date created: 2012-10-02 11:00:00
Date modified: 2015-05-08 01:13:48

LC Cutinase mutant F125Y



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K936033_sequence (Version 1)

Description

Mutant of the Polyetheylene Terephtalate degrading protein LC Cutinase (Bba_K936000). The phenylalanine at the 125th amino acid position is changed to a tyrosine. This part was created using site directed mutagenesis to change a "TTC" codon to a "TAT" codon.

Notes

Eliminated BioBrick restriction sites and added a TAA double stop.

Source

Modified from the paper Isolation of a Novel Cutinase Homolog with Polyethylene Terephthalate-Degrading Activity from Leaf-Branch Compost by Using a Metagenomic Approach by Sulaiman et al.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3294458/

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BBa_K936033/1