BBa_K936033

BBa_K936033 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K936033

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sergio Castro Waqued

Date created: 2012-10-02 11:00:00

Date modified: 2015-05-08 01:13:48

LC Cutinase mutant F125Y

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K936033_sequence (Version 1)

Description

Mutant of the Polyetheylene Terephtalate degrading protein LC Cutinase (Bba_K936000). The phenylalanine at the 125th amino acid position is changed to a tyrosine. This part was created using site directed mutagenesis to change a "TTC" codon to a "TAT" codon.

Notes

Eliminated BioBrick restriction sites and added a TAA double stop.

Source

Modified from the paper Isolation of a Novel Cutinase Homolog with Polyethylene Terephthalate-Degrading Activity from Leaf-Branch Compost by Using a Metagenomic Approach by Sulaiman et al.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3294458/

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K936033/1

Member of these Collections

• iGEM Parts Registry