| Types | DnaRegion
|
| Roles | sequence_feature
Other
|
| Sequences | BBa_K940000_sequence (Version 1)
|
Description
This part is a promoter for ALDH1A1,an enzyme involved in alcohol metabolism. Nucleotide sequences were analysed on NCBI. Sequences were selected by determining the start of the protein coding region and then taking the previous 1000 bases which included the TAATAA box. The sequences selected were compared with commercial sequences. Additionally overlap between the selected sequence and sequences on the Eukaryotic Promoter database (EPD) was confirmed. ALDH sequence was selected to exclude BioBrick restriction sites.
Notes
The sequence identified did not have any illegal sites, thus the primers were designed so as to have the sequence flanked with biobrick prefix and suffix.
Source
Genomic sequence of Homosapiens.
