BBa_K942003

BBa_K942003 Version 1

Component

This part has been discontinued.

Please consider using BBa_K942018 as a replacement.

Source: http://parts.igem.org/Part:BBa_K942003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Luis Mario Leal Garza

Date created: 2012-09-25 11:00:00

Date modified: 2015-05-08 01:13:48

SS+B.P.S.

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K942003_sequence (Version 1)

Description

Using this part after the 3??? of a yeast promoter and before the 5??? of any CDS, the user can make a single construct that can yield protein expression in both, E.coli (DE3 strains) and yeast. In our case we used the AOX1 promoter and the CDS for Der f 2 for expression in BL21 STAR, Rossettagami and P.pastoris.

About Safety
This part presents no biosecurity issues apart that any construct after the 3??? of this part could be expressed both in yeast and in bacteria.

Notes

Encrypted in non-translatable sequence after the SS, the T7 promoter (BBa_I712074) and a RBS promote the bacterial expression of the protein. This last feature lets the user transform the same construct for bacterial intracellular expression and extracellular yeast expression. Use this part after the 3??? of a yeast promoter and before the 5??? of any CDS.

Source

This part is a mix from the SS of α-mating factor pre-sequence and a consensus non-translational yeast mRNA sequence.

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• isReplacedBy: BBa_K942018/1
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K942003/1

Member of these Collections

• iGEM Parts Registry