BBa_K942004

BBa_K942004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K942004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Luis Mario Leal Garza

Date created: 2012-09-25 11:00:00

Date modified: 2015-05-08 01:13:48

Shuttle Sequence (SS+B.P.S.)+Der f 2+His

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K942004_sequence (Version 1)

Description

Der f 2 is a major allergen from Dermatophagoides farinae, although its biological function in the dust mite has not been well discovered yet. It comes flanked with the α-mating factor pre-sequence for protein secretion.

This protein has an estimated weight of 16.6kDa. It has a folding similar to that of an immunoglobulin and also has a suspected antibacterial activity, yet it has been expressed previously in E.coli. We recommend its affinity purification using the 6x His tag. (Liu Z, 2007)

About Safety
The Der f 2 protein is a major allergen from the dust mite, so any contact with cells transformed with this part should be done with appropriate gloves. Also we recommend that the use must make sure he/she is not allergic to dust mite. The secretion of this protein makes this part more hazardous so you would like to have extra precaution to prevent any leak from your samples.

Notes

This composite part has a secretion signal (SS) for an easier purification, maxed out using affinity purification with the C-terminal 6x His tag. As the SS is functional only in yeast, the codon optimization for this sequence was done for a yeast-like codon bias. Also, encrypted in non-translatable sequence after the SS, the T7 promoter (BBa_I712074) and a RBS promote the bacterial expression of the protein. This last feature lets the user transform the same construct for bacterial intracellular expression and extracellular yeast expression.

Source

This part is a mix from the SS of α-mating factor pre-sequence, Der f 2 allergen from Dermatophagoides farinae, and a consensus non-translational yeast mRNA sequence. (Uniprot:Q5TIW0)

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K942004/1

Member of these Collections

• iGEM Parts Registry