Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Luis Mario Leal Garza
Date created: 2012-09-25 11:00:00
Date modified: 2015-05-08 01:13:48
SS+Api m 6+His
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K942007_sequence (Version 1) |
Description
The Api m 6 allergen native from Apis miellifera, is an allergen from the bee venom. Not only it acts as an allergen but it also contains a Trypsin-Inhibitor Domain. The secretion signal (SS) from α-mating factor pre-sequence is included for this construct also for extracellular secretion. (Peiren N., 2006)This allergen has a molecular weight of 8.4kDa and is better visualized in a SDS-PAGE with Tris-Tricine gel buffer, because of its low molecular weight. It can be purified by affinity with its 6xHis tag. (Gasteiger E., 2005)
Safety
The Api m 6 protein is an allergen from the bee venom, so any contact with cells transformed with this part should be done with appropriate gloves. Also we recommend that the use must make sure he/she is not allergic to bee venom. The secretion of this protein makes this part more hazardous so you would like to have extra precaution to prevent any leak from your samples.