BBa_K942011

BBa_K942011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K942011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Luis Mario Leal Garza

Date created: 2012-09-25 11:00:00

Date modified: 2016-01-27 10:45:02

RiAFP+His

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K942011_sequence (Version 1)

Description

The Raghium inquisitor Anti Freeze Protein (RiAFP) is a 137 AA protein that prevents the growth of ice crystals. This protein is produced natively in the beetle Raghium inquisitor. (Kristiansen E, 2011)

About Safety
The RiAFP has no reported toxicity or hazardousness, but its important to remember to have the basic biosafety precautions using a biosecurity chamber when handling any transformed strains.

Notes

This part was designed by adding a 6xHis tag for affinity purification to the anti freeze protein from Raghium inquisitor. This part was codon optimized for its expression in E.coli.

Source

Is produced natively in the beetle Raghium inquisitor. (Kristiansen E, 2011)

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K942011/1

Member of these Collections

• //collections/antifreeze
• iGEM Parts Registry