BBa_K950010

BBa_K950010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K950010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jakob Matthes

Date created: 2012-09-12 11:00:00

Date modified: 2015-05-08 01:13:49

pRS315 shuttle vector

Details

• Types: DnaRegion
• Roles: plasmid; Plasmid
• Sequences: BBa_K950010_sequence (Version 1)

Description

pRS315 is a Saccharomyces/E. coli shuttle vector. The multiple cloning site has XbaI and SpeI restriction sites. EcoRI and PstI cannot be used with this plasmid. It contains the LEU2 gene for selection.

Notes

EcoRI and SpeI restriction cannot be used with this vector.

Source

http://genome-www.stanford.edu/vectordb/vector_descrip/PRS315.html

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LEU2 beta-isopropylmalate	663,1757	feature/cds, CDS
F1 origin of replication	2698,2797	sequence_feature, feature/misc
lacZ	2931,3268	feature/cds, CDS
T7 promoter	3091,3116	feature/promoter, promoter
mcs	3124,3225	sequence_feature, feature/misc
XbaI	3148,3153	sequence_feature, feature/misc
SpeI	3154,3159	feature/misc, sequence_feature
T3 promoter	3243,3262	feature/promoter, promoter
PBM1 origin of replication	3686,3686	sequence_feature, feature/misc
bla beta-lactamase, ampR ampicillin	4444,5304	feature/cds, CDS

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K950010/1

Member of these Collections

• iGEM Parts Registry