BBa_K952000

BBa_K952000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K952000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Steven Neuhaus
Date created: 2012-09-26 11:00:00
Date modified: 2015-05-08 01:13:49

Phytochrome FphA - first 753 amino acids (Frieburg)



Types
DnaRegion

Roles
Protein_Domain

polypeptide_domain

Sequences BBa_K952000_sequence (Version 1)

Description

Sensory domain of phytochrome FphA which has been codon optimized for expression in E. coli and conforms to RFC25 (Freiburg Standard) in order to allow for the fusion of other protein domains N- and C- terminally.
Note that in order for the sensor to function, FphA must bind a chromophore (biliverdin IXalpha (BV) acts as its natural chromophore). The chromophore can either be externally added to the protein, or can be endogenously created through expression of heme oxygenase.
Illumination with saturated red light (636 nm) results in formation of the Pfr form of the phytochrome (Amax 754nm), which can be converted back into the ground Pr form (Amax 707 nm) through illumination with far-red light (750 nm) (Brandt,S., Frankenberg-Dinkel,N., et. al. (2008) J. Biol. Chem. 283 (50), 34605-34614)

Notes

Cut sites had to be removed from the source gene in order to comply with Biobrick standards.

Source

This part comes from the fungus Aspergillus nidulans, although it has been codon optimized for expression in E. coli. See http://www.ncbi.nlm.nih.gov/nuccore/167987798

Sequence Annotation Location Component / Role(s)
C195, biliverdin IX􏰃 binding residue
583,585
conserved_region feature/conserved
igem#sampleStatus
It's complicated
igem#status
Planning
 
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user/james
 
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user/myers
 
synbiohub#topLevel
BBa_K952000/1