BBa_K952001

BBa_K952001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K952001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Saimon Sharif

Date created: 2012-09-30 11:00:00

Date modified: 2015-05-08 01:13:49

PfixK2-Bacteriophage Lysis Cassette

Details

• Types: DnaRegion
• Roles: Intermediate; engineered_region
• Sequences: BBa_K952001_sequence (Version 1)

Description

This part contains the FixK2 promoter (BBa_K592006), to which phospho-FixJ binds to, as an upstream part. The downstream part is a gene cassette which(BBa_K124017), when activated by an inducer, begins to produce proteins which lyse the cell. This composite part can be used with in conjunction with FixJ and YF1, a blue-light sensing protein, to cause cell death when the cells are exposed to blue-light.

Notes

N/A

Source

PfixK2 was obtained from the 2011 Uppsala-Sweden team; it is available as BBa_K592006 in the registry. The bacteriophage lysis cassette was obtained from the 2012 plates (BBa_K124017).

Components

Access	Instance	Definition
public	BBa_K592006	BBa_K592006
public	BBa_K124017	BBa_K124017

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K592006	1,250	BBa_K592006
BBa_K124017	257,1513	BBa_K124017

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K952001/1

Member of these Collections

• iGEM Parts Registry