BBa_K953000

BBa_K953000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K953000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ryan Kenny

Date created: 2012-09-19 11:00:00

Date modified: 2015-05-08 01:13:49

Heme Oxygenase with T7 & RBS (E. coli Codon Optimised)

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K953000_sequence (Version 1)

Description

E. coli codon optimised Synechocystis Heme Oxygenase with a Ribosome Binding Site (RBS) and T7 promoter (Part BBa1719005, oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration.

Notes

GC rich regions, which formed hairpin loops in synthesis, were removed. Restriction sites were removed to conform to biobrick standards.

Source

The part was codon optimsed (http://www.bioinformatics.org/sms2/rev_trans.html) from Synechocystis and assembled using Gibson Assembly. Select GC rich regions were removed.

Components

Access	Instance	Definition
public	BBa_I712074	BBa_I712074
public	BBa_K953004	BBa_K953004

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I712074	1,46	BBa_I712074
BBa_K953004	47,794	BBa_K953004

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K953000/1

Member of these Collections

• iGEM 2018 Distribution Plate 6
• iGEM 2018 Distribution Plate 6 Well 10C
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM 2019 Distribution Plate 4
• iGEM 2019 Distribution Plate 4 Well 10C
• iGEM 2019 Part Library
• iGEM 2019 Plasmid pSB1C3
• iGEM Parts Registry