Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Winter
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:49
Agrobacterium tumefaciens Bacteriophytochrome with RBS (E. coli Codon Optimised)
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K953002_sequence (Version 1) |
Description
Agrobacterium tumefaciens bacteriophytochrome, codon optimised for expression in E. coli. In the presence of biliverdin the bacteriophytochrome will undergo a conformation change. This complex can then absorb red light(620-750 nm) to excite the complex and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time.As E. coli does not produce biliverdin, heme oxygenase (Part BBa_K953000) can be coupled with this part to activate the oxidation of heme to produce biliverdin or by the addition of biliverdin to the cells.
Notes
Optimised for expression in E. coliSource
Agrobacterium tumefaciens| Sequence Annotation | Location | Component / Role(s) |
| RBS Start Codon Bacteriophytochrome Stop BBa_K953002 | 1,22 23,25 26,2230 2231,2236 1,2236 | feature/rbs ribosome_entry_site feature/start start_codon feature/cds CDS stop_codon feature/stop feature/BioBrick engineered_region |