Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Dubow
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:49
Agrobacterium tumefaciens Bacteriophytochrome with T7 & RBS (E. coli Codon Optimised)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K953003_sequence (Version 1) |
Description
Agrobacterium tumefaciens bacteriophytochrome, codon optimised for expression in E. coli. In the presence of biliverdin the bacteriophytochrome will undergo a conformation change. This complex can then absorb red light (620-750 nm) to excite the complex and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase (Part BBa_K953004) can be coupled with this part to activate the oxidation of heme to produce biliverdin or by the addition of biliverdin to the cells.This part contains a T7 Promotor.
Notes
Codon optimisation for E. coli, whilst staying within the GC constraints of gibson assembly.Source
Gibson Assembly| Access | Instance | Definition |
| public public | BBa_I712074 BBa_K953002 | BBa_I712074 BBa_K953002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I712074 BBa_K953002 | 1,46 47,2282 | BBa_I712074 BBa_K953002 |