Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dylan Patrick Webster
Date created: 2012-07-27 11:00:00
Date modified: 2015-05-08 01:13:49
Salicylate inducible mtrB with cut sites flanking RBS
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K957002_sequence (Version 1) |
Description
The purpose of this part is to upregulate mtrB expression in response to salicylate. When expressed in a Shewanella strain lacking mtrB on the chromosome, this composite part will function as a component of a biosensor. Specifically, when such a complemented strain is inoculated in a microbial electrochemical system, current output will increase in response to salicylate. If coupled to a the expression of a BioBrick part that degrades naphthalene to salicylate, this part will function as part of a naphthalene biosensor.Notes
Constructed using non-BioBrick AscI cutsite of BBa_K957000.Source
mtrB is originally derived from the mtrCAB operon of Shewanella oneidensis MR-1; BioBrick part BBa_K098994 was used as DNA source.The salicylate sensitive region was isolated via PCR from a plasmid containing BBa_K228227, designed by PKU_Beijing 2009.
| Sequence Annotation | Location | Component / Role(s) |
| nahR BBa_K228004 Pr promoter pSal BBa_K098989 mtrB silent mutation, original base G KpnI cut site AscI cut site BamHI cut site mtrB rbs | 1,903 1,1052 904,993 982,1050 1079,3172 1079,3172 3143,3143 3202,3207 1056,1063 1073,1078 1066,1071 | feature/cds CDS feature/BioBrick engineered_region feature/promoter promoter feature/promoter promoter feature/BioBrick engineered_region CDS feature/cds feature/mutation sequence_alteration sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/rbs ribosome_entry_site |