Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dylan Patrick Webster
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:49
Salicylate inducible mtrB
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K957003_sequence (Version 1) |
Description
The purpose of this part is to upregulate mtrB expression in response to salicylate. When expressed in a Shewanella strain lacking mtrB on the chromosome, this composite part will function as a component of a biosensor. Specifically, when such a complemented strain is inoculated in a microbial electrochemical system, current output will increase in response to salicylate. If coupled to the expression of a BioBrick part that degrades naphthalene to salicylate, this part will function as part of a naphthalene biosensor.Notes
Constructed using non-BioBrick AscI cutsite of BBa_K957001.Source
mtrB is originally derived from the mtrCAB operon of Shewanella oneidensis MR-1; BioBrick part BBa_K098994 was used as DNA source.The salicylate sensitive region was isolated via PCR from a plasmid containing BBa_K228227, designed by PKU_Beijing 2009.
| Sequence Annotation | Location | Component / Role(s) |
| K228004 nahR Pr Psal AscI cut site mtrB K098989 | 1,1052 1,903 904,993 982,1050 1054,1061 1088,3181 1088,3181 | engineered_region feature/BioBrick CDS feature/cds promoter feature/promoter promoter feature/promoter sequence_feature feature/misc feature/cds CDS feature/BioBrick engineered_region |