BBa_K957004

BBa_K957004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K957004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dylan Patrick Webster
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:49

nah Operon for degradation of naphthalene to salicylate



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K957004_sequence (Version 1)

Description

One of two positively regulated operons of the NAH7 plasmid from Pseudomonas putida which encode the enzymes for metabolism of naphthalene via salicylate. When used with a BioBrick generator responsive to salicylate via the NahR activator (e.g. BBa_K957002 and BBa_K957003), this operon can function as part of a whole-cell biosensor for naphthalene.

Notes

Three internal BioBrick cut sites (one NotI, two PstI) remain in this version of the part. However, the utility of the part is not restricted, since digestion with EcoRI and SpeI facilitates standard molecular cloning.

Source

From NAH7 plasmid of Pseudomonas putida G7 (provided by Dr. Gene Madsen, Cornell University Department of Microbiology).

Sequence Annotation Location Component / Role(s)
nahAa
nahAb
nahAc
nahAd
nahB
nahF
nahC
nahQ
nahE
nahD
PstI cut site
PstI cut site
NotI cut site
13,999
1143,1466
1528,2877
2892,3476
3546,4325
4371,5822
5849,6757
6854,7486
7535,8530
8795,9406
1867,1872
5862,5867
8165,8172
CDS feature/cds
CDS feature/cds
CDS feature/cds
CDS feature/cds
CDS feature/cds
feature/cds CDS
feature/cds CDS
CDS feature/cds
CDS feature/cds
CDS feature/cds
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K957004/1