Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dylan Patrick Webster
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:49
nah Operon for degradation of naphthalene to salicylate
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K957004_sequence (Version 1) |
Description
One of two positively regulated operons of the NAH7 plasmid from Pseudomonas putida which encode the enzymes for metabolism of naphthalene via salicylate. When used with a BioBrick generator responsive to salicylate via the NahR activator (e.g. BBa_K957002 and BBa_K957003), this operon can function as part of a whole-cell biosensor for naphthalene.Notes
Three internal BioBrick cut sites (one NotI, two PstI) remain in this version of the part. However, the utility of the part is not restricted, since digestion with EcoRI and SpeI facilitates standard molecular cloning.Source
From NAH7 plasmid of Pseudomonas putida G7 (provided by Dr. Gene Madsen, Cornell University Department of Microbiology).| Sequence Annotation | Location | Component / Role(s) |
| nahAa nahAb nahAc nahAd nahB nahF nahC nahQ nahE nahD PstI cut site PstI cut site NotI cut site | 13,999 1143,1466 1528,2877 2892,3476 3546,4325 4371,5822 5849,6757 6854,7486 7535,8530 8795,9406 1867,1872 5862,5867 8165,8172 | CDS feature/cds CDS feature/cds CDS feature/cds CDS feature/cds CDS feature/cds feature/cds CDS feature/cds CDS CDS feature/cds CDS feature/cds CDS feature/cds sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc |