Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dylan Patrick Webster
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:49
Constitutive expression of nah Operon for degradation of naphthalene to salicylate
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K957005_sequence (Version 1) |
Description
One of two positively regulated operons of the NAH7 plasmid from Pseudomonas putida which encode the enzymes for metabolism of naphthalene via salicylate. When used with a BioBrick generator responsive to salicylate via the NahR activator (e.g. BBa_K957002 and BBa_K957003), this operon can function as part of a whole-cell biosensor for naphthalene.Notes
Three internal BioBrick cut sites (one NotI, two PstI) remain in this version of the part. However, the utility of the part is not restricted, since digestion with EcoRI and SpeI facilitates standard molecular cloning.Source
From NAH7 plasmid of Pseudomonas putida G7 (provided by Dr. Gene Madsen, Cornell University Department of Microbiology).| Access | Instance | Definition |
| public public | BBa_J23114 BBa_K957004 | BBa_J23114 BBa_K957004 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23114 BBa_K957004 | 1,35 44,9452 | BBa_J23114 BBa_K957004 |