Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dylan Patrick Webster
Date created: 2012-10-27 11:00:00
Date modified: 2015-05-08 01:13:49
Naphthalene Dioxygenase (From Pseudomonas NAH7 plasmid)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K957015_sequence (Version 1) |
Description
When the nah operon from the NAH7 Pseudomonas plasmid is heterologously expressed in E. coli, the cells have the ability to synthesize indigo from tryptophan [1].E. coli contains the enzyme tryptophanase, which splits the indole group from the peptide backbone. Naphthalene dioxygenase, from the nah operon, then creates a compound which will spontaneously eliminate water and be air oxidized into indigo as the final product.
[1] Ensley, B., Ratzkin, B., Osslund, T., Simon, M., Wackett, L., & Gibson, D. (1983). Expression of naphthalene oxidation genes in Escherichia coli results in the biosynthesis of indigo. Science, 222(4620), 167-9.
Notes
Internal PstI cutsite deleted w/ mutagenic PCR.Source
Psueomonas putida NAH7 plasmid.Provided by Dr. Eugene L. Madsen, Cornell University Department of Microbiology