Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eric Kessler
Date created: 2013-06-05 11:00:00
Date modified: 2015-05-08 01:13:50
Pyruvate Kinase derived from Rabbit Muscle
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K977000_sequence (Version 1) |
Description
This part is the 1569 bp long coding sequence of a pyruvate kinase gene variant derived from rabbit muscle (Oryctolagus cuniculus pyruvate kinase, muscle (PKM), transcript variant 1, mRNA)GenBank link - http://www.ncbi.nlm.nih.gov/nucleotide/307548865?report=genbank&log$=nuclalign&blast_rank=1&RID=V0YWBB4E01R
Pyruvate kinase is a key regulatory enzyme within glycolysis that removes a phosphate from phosphoenolpyruvate (PEP) adding it to ADP in the production of ATP and pyruvate. The enzyme is allosterically regulated by both inhibitory and excitatory substances.
We are attempting to use pyruvate kinase to enhance the downstream metabolic production of alkanes.
Notes
The original pyruvate kinase gene contained two PstI restriction sites beginning at bp 550 and 908. These restriction sites were removed by either 1) site directed mutagenesis or 2) the purchasing of designed oligos that were stitched together using Gibson Assembly.Source
The part was derived from an existing previously constructed plamid in E. coli, carrying the rabbit muscle pyruvate kinase.| Sequence Annotation | Location | Component / Role(s) |
| ATG Start Codon Pyruvate Kinase Gene Site Directed Mutation CTG to CTT Site Directed Mutation GCA to GCG ATT ATT Stop Codons | 1,3 1,1601 549,551 909,911 1596,1601 | start_codon feature/start CDS feature/cds sequence_feature feature/misc sequence_feature feature/misc stop_codon feature/stop |