Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Isaac Ward
Date created: 2013-06-20 11:00:00
Date modified: 2015-05-08 01:13:50
Oxytocin-Neurophysin1
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K988002_sequence (Version 1) |
Description
This Part was developed from the Homosapien sapien (human) coding sequence for Oxytocin-Neurophysin1(1) the original signal sequence was replaced with a new (PelB) signal sequence (2) to send the protein to periplasmic space on and then be cleaved the 5' end this also allows for potential increased stability of the folded protein. A 6x-His tag was added for purification on the 3' end.(1)http://www.uniprot.org/uniprot/P01178
(2)http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2259416/
(3)http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0063442
Notes
The part was back translated from the protein sequence to ensure the right DNA sequence was obtained for the proper protein sequence.When adding the PelB signal sequence it was was very important to consider the cleavage site as the C-C disulfide bond was necessary to be translated, without a signal sequence the protein would have to start with a Methionine, with would disrupt the C-C disulfide bond.
the His tag was added to help with with protein Purification.
Source
This Part was developed from the Homosapien sapien coding sequence for Oxytocin-Neurophysin1| Sequence Annotation | Location | Component / Role(s) |
| Signal Sequence Oxytocin Neurophysin 1 6x Histidine tag & stop codon | 2,67 68,95 96,386 386,406 | sequence_feature feature/misc feature/protein CDS CDS feature/cds feature/tag tag |